Application Notes

Purification-free labeling in whole cell lysate and binding characterization by MST

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APPLICATION NOTE Monolith is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2017 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. NT-MO-32-01 nanotempertech.com References 1. Dominguez et al., p38 MAP kinase inhibitors: many are made, but few are chosen. Curr Opin Drug Discov Devel. 2005 Jul;8(4):421-30. 2. Davis et al., Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J. 2000 Oct 1;351(Pt 1):95-105. 3. Saxena et al., Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38. Application Note NT-MO-003 (2011). 4. Tschammer et al., One-step, purification-free and site-specific labeling of polyhistidine-tagged proteins for MST. Application Note NT-MO-29 (2016). 5. Cuenda et al., SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1. 1995 FEBS Lett 229-233.

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