Application Notes

One-step, purification-free and site-specific labeling of polyhistidine-tagged proteins for MST

Issue link: https://resources.nanotempertech.com/i/1050590

Contents of this Issue

Navigation

Page 7 of 7

APPLICATION NOTE Monolith is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2017 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. NT-MO-029-01 nanotempertech.com References 1. Ueda, E. K., Gout, P. W. & Morganti, L. Current and prospective applications of metal ion-protein binding. J Chromatogr A 988, 1-23 (2003). 2 Hochuli, E., Dobeli, H. & Schacher, A. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr 411, 177-184 (1987). 3 Gershon, P. D. & Khilko, S. Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector. J Immunol Methods 183, 65-76 (1995). 4 You, C. & Piehler, J. Multivalent chelators for spatially and temporally controlled protein functionalization. Anal Bioanal Chem 406, 3345-3357, doi:10.1007/s00216-014-7803-y (2014). 5 Huang, Z., Hwang, P., Watson, D. S., Cao, L. & Szoka Jr, F. C. Tris- nitrilotriacetic acids of subnanomolar affinity toward hexahistidine tagged molecules. Bioconjugate chemistry 20, 1667-1672 (2009). 6 Lata, S., Reichel, A., Brock, R., Tampé, R. & Piehler, J. High-affinity adaptors for switchable recognition of histidine-tagged proteins. Journal of the American Chemical Society 127, 10205-10215 (2005). 7 Kim, S. H., Jeyakumar, M. & Katzenellenbogen, J. A. Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling. Journal of the American Chemical Society 129, 13254-13264 (2007). 8 Lata, S., Gavutis, M., Tampé, R. & Piehler, J. Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation. Journal of the American Chemical Society 128, 2365-2372 (2006). 9 Terpe, K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied microbiology and biotechnology 60, 523-533 (2003). 10 Glembockyte, V., Lincoln, R. & Cosa, G. Cy3 Photoprotection Mediated by Ni2+ for Extended Single-Molecule Imaging: Old Tricks for New Techniques. Journal of the American Chemical Society 137, 1116-1122, doi:10.1021/ja509923e (2015). 11 Nordin, H., Jungnelius, M., Karlsson, R. & Karlsson, O. P. Kinetic studies of small molecule interactions with protein kinases using biosensor technology. Analytical biochemistry 340, 359-368, doi:10.1016/j.ab.2005.02.027 (2005). 12 Rohle, D. et al. An inhibitor of mutant IDH1 delays growth and promotes differentiation of glioma cells. Science 340, 626-630, doi:10.1126/science.1236062 (2013). 13 Walzer, S. A. et al. Crystal Structure of the Human Cytomegalovirus pUL50-pUL53 Core Nuclear Egress Complex Provides Insight into a Unique Assembly Scaffold for Virus-Host Protein Interactions. Journal of Biological Chemistry 290, 27452-27458, doi:10.1074/jbc. C115.686527 (2015).

Articles in this issue

Links on this page

view archives of Application Notes - One-step, purification-free and site-specific labeling of polyhistidine-tagged proteins for MST