Application Notes

Using MST to analyse the binding of nanobodies and Nanobody-Fc fusion proteins to human CD38

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3 Instrumentation The measurements were performed using a NanoTemper Monolith NT.115 instrument. References 1. Partida-Sanchez, S., L. Rivero-Nava, G. Shi, and F.E. Lund. 2007. CD38: an ecto-enzyme at the crossroads of innate and adaptive immune responses. Adv Exp Med Biol 590:171-183. 2. Malavasi, F., S. Deaglio, A. Funaro, E. Ferrero, A.L. Horenstein, E. Ortolan, T. Vaisitti, and S. Aydin. 2008. Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol Rev 88:841-886. 3. Hamers-Casterman, C., T. Atarhouch, S. Muyldermans, G. Robinson, C. Hamers, E.B. Songa, N. Bendahman, and R. Hamers. 1993. Naturally occurring antibodies devoid of light chains. Nature 363:446-448. 4. Saerens, D., G.H. Ghassabeh, and S. Muyldermans. 2008. Single-domain antibodies as building blocks for novel therapeutics. Curr Opin Pharmacol 8:600- 608. 5. Wesolowski, J., V. Alzogaray, J. Reyelt, M. Unger, K. Juarez, M. Urrutia, A. Cauerhff, W. Danquah, B. Rissiek, F. Scheuplein, N. Schwarz, S. Adriouch, O. Boyer, M. Seman, A. Licea, D.V. Serreze, F.A. Goldbaum, F. Haag, and F. Koch-Nolte. 2009. Single domain antibodies: promising experimental and therapeutic tools in infection and immunity. Med Microbiol Immunol 198:157-174. 6. Huang, L., S. Muyldermans, and D. Saerens. 2010. Nanobodies(R): proficient tools in diagnostics. Expert Rev Mol Diagn 10:777-785. 7. De Genst, E., K. Silence, K. Decanniere, K. Conrath, R. Loris, J. Kinne, S. Muyldermans, and L. Wyns. 2006. Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies. Proc Natl Acad Sci U S A 103:4586-4591. 8. Liu, Q., I.A. Kriksunov, R. Graeff, C. Munshi, H.C. Lee, and Q. Hao. 2005. Crystal structure of human CD38 extracellular domain. Structure 13:1331-1339. 9. Koch-Nolte, F., J. Reyelt, B. Schossow, N. Schwarz, F. Scheuplein, S. Rothenburg, F. Haag, V. Alzogaray, A. Cauerhff, and F.A. Goldbaum. 2007. Single domain antibodies from llama effectively and specifically block T cell ecto-ADP-ribosyltransferase ART2.2 in vivo. FASEB J 21:3490-3498. 10. Scheuplein, F., B. Rissiek, J.P. Driver, Y.G. Chen, F. Koch-Nolte, and D.V. Serreze. 2010. A recombinant heavy chain antibody approach blocks ART2 mediated deletion of an iNKT cell population that upon activation inhibits autoimmune diabetes. J Autoimmun 34:145-154. 11. Moran Jerabek-Willemsen, Christoph J. Wienken, Dieter Braun, Philipp Baaske and Stefan Duhr. Molecular Interaction Studies Using Microscale Thermophoresis. ASSAY and Drug Development Technologies, DOI:10.1089/adt.2011.0380 (2011) 12 Christoph J. Wienken, Philipp Baaske, Ulrich Rothbauer, Dieter Braun and Stefan Duhr .Protein Binding Assays in Biological Liquids using Microscale Thermophoresis. Nature Communications, DOI: 10.1038/ncomms1093 (2010) 13. Stefan Duhr and Dieter Braun. Why molecules move along a temperature gradient. PNAS 103, 19678–19682 (2006) © 2012 NanoTemper Technologies GmbH

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