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Characterizing protein stability, powerful approaches to study protein denaturation

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20 To make the purchase more affordable, consider asking for funding support from other groups at your institution who have a need and budget. Or, consider purchasing an entry-level instrument and plan to upgrade it at a later time. As with any other big purchase, once the financing is in place, the rest is relatively straightforward. Most technologies will require some form of installation, implementation, and training, but aside from occasional support and maintenance, your new instrument is ready to become an invaluable part of your workflow. As a researcher, chances are a critical piece of your experiments involves characterizing proteins. By measuring a protein's stability, you can gain new insights into the molecule's complex functions. Now that you're informed about what protein stability is and how it is important, you will know how to evaluate and purchase the appropriate tools or technologies for your lab. Resources for Protein Stability If you are interested in learning more about binding affinity and other bioanalytical screening methods, visit these resources: Conclusion Quality assessment and optimization of purified protein samples: why and how? https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-014-0180-6 Protein Stability: A Crystallographer's Perspective https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741188/ Guidelines on Stability Evaluation of Vaccines from the World Health Organization http://www.who.int/biologicals/publications/trs/areas/vaccines/stability/en/ Guideline for Industry: Quality of Biotechnological Products: Stability Testing of Biotechnological/Biological Products from the U.S. Food and Drug Administration https://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm073466.pdf

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