12
Differential
Scanning
Calorimetry
(DSC)
Thermofluor/
Differential
Scanning
Fluorimetry
(DSF)
Nano Differential
Scanning
Fluorimetry
(nanoDSF)
Circular
Dichroism
(CD)
Requires high amount of sample? Yes No No Yes
Label-free? Yes No Yes Yes
High-throughput? No Yes Yes No
Easy to use? No Yes Yes No
Measures chemical or
thermal unfolding?
Thermal Thermal Thermal
Thermal and
Chemical
Technologies for
characterizing protein stability
How do I evaluate
my research needs?
Pro Tip
There are times when one technology can't perform all of the measurements you need.
Sometimes, you might need to use separate instruments to measure chemical stability and
thermal stability. But how many pieces of technology does your lab really need? When deciding
whether to go with a best-in-class or most-in-one solution, here are the key considerations:
• Do I need it now or do I need it in the future?
• Can I afford everything I need right now?
• Can I get enough information from one to complete my research goals?
