This was observed for three distinct mAbs, suggesting that the very slow equilibration kinetics
observed for partially unfolded mAbs might be a universal property of mAbs.
Based on these results, we recommend to use long incubation times (overnight, at least 16 h) after
preparation of the chemical denaturation samples. The results also show that a just-in-time
preparation of samples prior to measuring chemical denaturation produces results which
do not reflect the true equilibrium state of the system and are thus not suited to derive ΔG
values. It is therefore advisable to de-couple sample preparation and measurement for mAb
chemical denaturation experiments.
Contact
NanoTemper Technologies
GmbH
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81369 Munich
Germany
Phone: +49 (0)89 4522895 0
Fax: +49 (0)89 4522895 60
info@nanotemper-technologies.com
http://www.nanotemper-technologies.com
NanoTemper® , nanoDSF®, and Prometheus® are
registered trademarks.
V01_2016-09-15
