Application Notes

nanoDSF: Label-free thermal unfolding assay of G protein-coupled receptors for compound screening and buffer composition optimization

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APPLICATION NOTE Prometheus is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2017 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. NT-PR-008-01 nanotempertech.com Table 3. Buff er composition and osmolality screen for human test-GPCR. Receptor was purifi ed in presence of 10 µM compound #17. Best buff er condition ∆Tm + 2.6 °C. Receptor-detergent micelles apparently prefer a high osmotic buff er and slightly alkaline buff er. References 1. Zhang et al., (2015) Mol Cells 38(10): 836-842 2. Ascherio et al., (2001) Ann Neurol 50(1): 56-63 3. De Lera Ruiz et al., (2014) J Med Chem 57(9): 3623-50 4. Alexandrov et al., (2008) Structure 16(3): 351-359 5. Liu et al., (2012) Science 337(6091): 232-6

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