Application Notes

Analysis of formulation-dependent colloidal and conformational stability of monoclonal antibodies

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6 References 1. Uchiyama, S., Liquid formulation for antibody drugs. Biochim Biophys Acta, 2014. 1844(11): p. 2041- 2052. 2. Brader, M.L., et al., Examination of thermal unfolding and aggregation profiles of a series of developable therapeutic monoclonal antibodies. Mol Pharm, 2015. 12(4): p. 1005-17. 3. Rouet, R., D. Lowe, and D. Christ, Stability engineering of the human antibody repertoire. FEBS Lett, 2014. 588(2): p. 269-77. 4. Tiller, K.E. and P.M. Tessier, Advances in Antibody Design. Annu Rev Biomed Eng, 2015. 5. Cerasoli, E., et al., Temperature denaturation and aggregation of a multi-domain protein (IgG1) investigated with an array of complementary biophysical methods. Anal Bioanal Chem, 2014. 406(26): p. 6577-86. 6. Menzen, T. and W. Friess, Temperature-ramped studies on the aggregation, unfolding, and interaction of a therapeutic monoclonal antibody. J Pharm Sci, 2014. 103(2): p. 445-55. 7. Menzen, T. and W. Friess, High-throughput melting-temperature analysis of a monoclonal antibody by differential scanning fluorimetry in the presence of surfactants. J Pharm Sci, 2013. 102(2): p. 415- 28. 8. Garidel, P.K., A.R.; Blech, M. , Orthogonal spectroscopic techniques for the early developability assessment of therapeutic protein candidates. Spectroscopy Europe, 2014. 26(4): p. 9-13. 9. Garidel, P., Right Angle Light Scattering protein thermostability screening: for research and development. Spectroscopy Europe, 2012. 24(1): p. 13-18.

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