7
76
74
72
ECHIP
8
7
6
5
pH
200
150
100
50
0
NaCl
Tm-50C/40min
BufferC = 30.0
Trehalose = 5.0
ECHIP
0
1
2
3
4
5
6
7
8
9
10
Trehalose
5.0 5.5 6.0 6.5 7.0 7.5 8.0
pH
Tm-50C/40min
70.4
70.8
71.2
71.6
72.0
72.4
72.8
73.2
73.6
74.4
74.8
75.6
76.0
76.4
NaCl = 50.0
BufferC = 20.0
Value Low Limit High Limit
76.74 76.12 77.36
pH=5.00 Trehalos=10.00
76
74
72
70
ECHIP
8
7
6
5
pH
10
8
6
4
2
0
Trehalose
Tm-50C/40min
NaCl = 100.0
BufferC = 30.0
A
D
C
B
E
