nanoDSF
Prometheus characterizes thermal and chemical
unfolding under native conditions using nanoDSF
technology. nanoDSF precisely measures the
intrinsic fluorescence of a protein while it's
being subjected to either chemical or thermal
denaturation. The fluorescence intensity at a
single wavelength (350 or 330 nm) or the F350/330
ratio are plotted against increasing temperature
or concentrations of a chemical denaturant to
determine the T
m
or C
m
of a protein.
Use it to
• Monitor protein purification
• Evaluate target-ligand complexes before affinity-
based screening
• Monitor stability in biologics workflow during
formulation, developability, production, and
manufacturing
Fluorescence
intensity
(A.U.)
Wavelength (nm)
310 320 330 340 350 360 370
Temperature or denaturant
Fluorescence
ratio
unfolded
folded
T
m
/C
m
