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Site-specific labeling of antibodies for MicroScale Thermophoresis

Site-specific conjugation of a dye to antibodies is useful for producing highly homogeneous material for use in downstream affinity experiments. This application note describes a labeling procedure that combines the selective reduction of antibody interchain disulfides and their labeling with maleimide-based chemistry. Different antibodies conjugated by this approach yield high-quality MST affinity data for interactions with both antigens as well as Fc receptors.

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Purification-free labeling in whole cell lysate and binding characterization by MST
Purification-free labeling in whole cell lysate and binding characterization by MST

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Determination of low-picomolar affinities of sgRNAs and crRNA/tracrRNAs for Cas9
Determination of low-picomolar affinities of sgRNAs and crRNA/tracrRNAs for Cas9

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